* In addition to our industry-leading data quality, Illumina offers integrated mRNA-Seq workflows that simplify the entire process, from library preparation to data analysis and biological interpretation. Calculator to help determine the reagents and sequencing runs needed to arrive at desired 16s & ITS rRNA Sequencing; Metagenomic Sequencing; Microbial Whole-Genome Sequencing; we strive to meet this challenge. The Illumina Community. For specific trademark information, see www.illumina.com/company/legal.html. MiSeq Reagent Kits v2 are available in standard, micro, and nano configurations, enabling alignment to specific project requirements and scalable levels of output. Developing and automating workflows for analyzing, processing, and sharing genomic data among researchers and clinicians. This process translates into sequencing hundreds to thousands of genes at one time. The input requirement for DNA and RNA ranges For other applications, such as expression profiling or counting studies, shorter reads are sufficient and more cost-effective than longer ones. Sequencing reagent performance depends on thawing technique, thawed storage time, and storage conditions. Not for import or sale to the Australian general public. The Illumina Free Adapter Blocking Reagent is an optional reagent that can be used to treat most types of sequencing libraries to reduce index hopping levels. Tax Reg: 105-87-87282 | Now using 16s rRNA training set 18. Now using 16s rRNA training set 18. Illumina sequencing and array technologies fuel advancements in life science research, translational and consumer genomics, and molecular diagnostics. & Pipeline Setup, Sequencing Data With paired-end sequencing, after a DNA fragment is read from one end, the process starts again in the other direction. Achieve consistent, reproducible library prep on AmpliSeq for Illumina panels with automation solutions from our trusted partners. Bio-IT Platform, TruSight Support Center / 16S Metagenomic Sequencing Library Preparation. Enable comprehensive genomic profiling with accurate and comprehensive homologous recombination deficiency assessment, Discover novel trait and disease associations with optimized tag SNPs and functional exonic content at an attractive price, All Software & Informatics Select from over 5000 pretested genes relevant for inherited disease research. The Illumina Free Adapter Blocking Reagent is an optional reagent that can be used to treat most types of sequencing libraries to reduce index hopping levels. Illumina offers integrated workflows that simplify sequencing, from library preparation to data analysis. The AmpliSeq for Illumina solution offers a highly multiplexed polymerase chain reaction (PCR)-based workflow for use with targets ranging from a few to hundreds in a single run. Discuss best practices, troubleshoot, and learn about how others are using Illumina sequencers, library preparation kits, and automated data analysis to fuel their research. This technology was subsequently referred to as sequencing by synthesis technology, or SBS. This technology was subsequently referred to as sequencing by synthesis technology, or SBS. Choosing the right sequencing read length depends on your sample type, application, and coverage requirements. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics. Oligonucleotide (oligo) sequences of Illumina adapters used in AmpliSeq, Nextera, TruSeq, and 16s & ITS rRNA Sequencing; Metagenomic Sequencing; Microbial Whole-Genome Sequencing; we strive to meet this challenge. Experience our simplest workflow yet with dry instrumentation, easier run set up, and 6x faster secondary analysis. Address of host server location: 5200 Illumina Way, San Diego, CA 92122 U.S.A. All trademarks are the property of Illumina, Inc. or their respective owners. These resources cover key topics in NGS and are designed to help you plan your first experiment. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. 2. Join Now Shotgun metagenomic sequencing allows researchers to comprehensively sample all genes in all organisms present in a given complex sample. of large targets using multiple primer pools to create overlapping amplicons. Targeted RNA expression panel investigating 395 genes involved in tumor-immune system interactions. MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. | Terms and conditions apply. & Pipeline Setup, Sequencing Data Sequence Hub, BaseSpace Fill out the form below to contact a sales representative to see if you are eligible. Variant Interpreter, MyIllumina Interpretation, Certificates (CofC, CofA) and Master Lot Sheets, AmpliSeq for Illumina Cancer Hotspot Panel v2, AmpliSeq for Illumina Comprehensive Cancer Panel, Breast Cancer Target Identification with High-Throughput NGS, The Complex World of Pan-Cancer Biomarkers, Microbiome Studies Help Refine Drug Discovery, Identifying Multidrug-Resistant Tuberculosis Strains, Investigating the Mysterious World of Microbes, IDbyDNA Partnership on NGS Infectious Disease Solutions, Infinium iSelect Custom Genotyping BeadChips, 2020 Agricultural Greater Good Grant Winner, 2019 Agricultural Greater Good Grant Winner, Gene Target Identification & Pathway Analysis, TruSeq Methyl Capture EPIC Library Prep Kit, Genetic Contributions of Cognitive Control, Challenges and Potential of NGS in Oncology Testing, Partnerships Catalyze Patient Access to Genomic Testing, Patients with Challenging Cancers to Benefit from Sequencing, NIPT vs Traditional Aneuploidy Screening Methods, SNP Array Identifies Inherited Genetic Disorder Contributing to IVF Failures, NIPT Delivers Sigh of Relief to Expectant Mother, Education is Key to Noninvasive Prenatal Testing, Study Takes a Look at Fetal Chromosomal Abnormalities, Rare Disease Variants in Infants with Undiagnosed Disease, A Genetic Data Matchmaking Service for Researchers, Using NGS to Study Rare Undiagnosed Genetic Disease, Progress for Patients with Rare and Undiagnosed Genetic Diseases, AmpliSeq for Illumina Immune Response Panel, TruSeq Targeted RNA Expression Library Prep Kits, Get faster cycle times, longer reads and more output from improved chemistry, Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit, Extend your read lengths with the 500-cycle kit, Choose the perfect number of cycles for your application (50, 300, or 500). 2022 Illumina, Inc. All rights reserved. At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. Available for human and other species. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. Discuss best practices, troubleshoot, and learn about how others are using Illumina sequencers, library preparation kits, and automated data analysis to fuel their research. input per pool. Keep samples and data in house, for more control and ability to build a database; A future-proof assay with emerging biomarkers already included; Learn more about the TruSight Oncology Product Family Illumina sequencing and array technologies fuel advancements in life science research, translational and consumer genomics, and molecular diagnostics. Collaborate with Illumina moderators, customers, and developers. 16s & ITS rRNA Sequencing; Metagenomic Sequencing; Microbial Whole-Genome Sequencing; Microbial Transcriptomics; Infectious Diseases. Products, DRAGEN v4.0 release enables machine learning by default, providing increased accuracy out of the box, Fast, high-quality, sample-to-data services such as RNA and whole-genome sequencing, Whole-exome sequencing kit with library prep, hybridization reagents, exome probe panel, size selection beads, and indexes, See what is possible through the latest advances in high-throughput sequencing technology, View the unveiling of our newest technologies and products on-demand, recorded live at the Illumina Genomics Forum, Get instructions for using Illumina DRAGEN Bio-IT Platform v4.0, A campus lab sequences dust from vacuum bags to understand the variants and viral load of SARS-CoV-2 and other viruses, Mapping genetic diversity to identify where confiscated gorillas come from and boost survival rates, Explore the advantages of NGS for analysis of gene expression, gene regulation, and methylation, The NovaSeq 6000Dx is our first IVD-compliant high-throughput sequencing instrument for the clinical lab. The performance of mNGS testing of 182 body fluids from 160 patients with acute illness was evaluated using two sequencing platforms in comparison to microbiological testing using culture, 16S bacterial PCR and/or 28S-internal The Illumina Free Adapter Blocking Reagent is an optional reagent that can be used to treat most types of sequencing libraries to reduce index hopping levels. Metagenomics is the study of genetic material recovered directly from environmental or clinical samples. & Pipeline Setup, Sequencing Data The AmpliSeq for Illumina solution offers a highly multiplexed Get instructions for sharing your desktop while working with Technical Support. Products, DRAGEN v4.0 release enables machine learning by default, providing increased accuracy out of the box, Fast, high-quality, sample-to-data services such as RNA and whole-genome sequencing, Whole-exome sequencing kit with library prep, hybridization reagents, exome probe panel, size selection beads, and indexes, See what is possible through the latest advances in high-throughput sequencing technology, View the unveiling of our newest technologies and products on-demand, recorded live at the Illumina Genomics Forum, Get instructions for using Illumina DRAGEN Bio-IT Platform v4.0, A campus lab sequences dust from vacuum bags to understand the variants and viral load of SARS-CoV-2 and other viruses, Mapping genetic diversity to identify where confiscated gorillas come from and boost survival rates, Explore the advantages of NGS for analysis of gene expression, gene regulation, and methylation, The NovaSeq 6000Dx is our first IVD-compliant high-throughput sequencing instrument for the clinical lab. For specific trademark information, see www.illumina.com/company/legal.html. Fast, high-quality, sample-to-data next-generation sequencing services, See what is possible through the latest advances in high-throughput sequencing technology, Empower the next generation of scientists and engineers through educational opportunities and resources, Upgrade your MiSeqDx instrument from Windows 7 to Windows 10, Enable comprehensive genomic profiling with accurate and comprehensive homologous recombination deficiency assessment, A campus lab sequences dust from vacuum bags to understand the variants and viral load of SARS-CoV-2 and other viruses, Mapping genetic diversity to identify where confiscated gorillas come from and boost survival rates, Explore the advantages of NGS for analysis of gene expression, gene regulation, and methylation, The NovaSeq 6000Dx is our first IVD-compliant high-throughput sequencing instrument for the clinical lab. Oligonucleotide (oligo) sequences of Illumina adapters used in AmpliSeq, Nextera, TruSeq, and 16s & ITS rRNA Sequencing; Metagenomic Sequencing; Microbial Whole-Genome Sequencing; we strive to meet this challenge. rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. Metagenomics is the study of genetic material recovered directly from environmental or clinical samples. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics. Illumina sequencing and array technologies fuel advancements in life science research, translational and consumer genomics, and molecular diagnostics. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. Made-to-order panels use coordinates you provide to target genomic regions of interest. A series of creative discussions in the lab and at a local pub during the summer of 1997 sparked ideas surrounding the use of clonal arrays and massively parallel sequencing of short reads using solid phase sequencing by reversible terminators. Retailer Reg: 2019--2018 | For specific trademark information, see www.illumina.com/company/legal.html. Apps, DRAGEN Not for import or sale to the Australian general public. Customer Dashboard, Infrastructure Not for use in diagnostic procedures (except as specifically noted). The AmpliSeq for Illumina solution offers a highly multiplexed polymerase chain reaction (PCR)-based workflow for use with targets ranging from a few to hundreds in a single run. Reagents for 24 reactions, resulting in fast amplicon library preparation with AmpliSeq panels. TruSeqIndexPlateFixtureKit(reusable) Illumina,catalog# FC1301005 [Optional]2100BioanalyzerDesktopSystem Agilent,part # G2940CA [Optional]AgilentDNA1000Kit Agilent,part # 50671504 [Optional]HighSpeedMicroPlateShaker VWR,catalog#13500890(110V/120V) or VWR,catalog#14216214(230V) DualIndexingPrinciple Looking for other types of cancer research panels? Species compatibility varies. Illumina offers integrated workflows that simplify sequencing, from library preparation to data analysis. This product is also available as an Illumina Advantage (TG) product. Improved Q30 score, support for UMIs, extended shelf life, and support for Illumina DNA PCR-Free Library Prep, Discover novel trait and disease associations with optimized tag SNPs and functional exonic content at an attractive price. Analysis, Biological Data Next-generation sequencing generates masses of DNA sequencing data, and is both less expensive and less time-consuming than traditional Sanger sequencing. Retailer Reg: 2019--2018 | AmpliSeq for Illumina is a suite of AmpliSeq chemistry products that are compatible with Illumina next-generation sequencing platforms. Not for import or sale to the Australian general public. Certificates (CofC, CofA) and Master Lot Sheets, AmpliSeq for Illumina Cancer Hotspot Panel v2, AmpliSeq for Illumina Comprehensive Cancer Panel, Breast Cancer Target Identification with High-Throughput NGS, The Complex World of Pan-Cancer Biomarkers, Microbiome Studies Help Refine Drug Discovery, Identifying Multidrug-Resistant Tuberculosis Strains, Investigating the Mysterious World of Microbes, IDbyDNA Partnership on NGS Infectious Disease Solutions, Infinium iSelect Custom Genotyping BeadChips, 2020 Agricultural Greater Good Grant Winner, 2019 Agricultural Greater Good Grant Winner, Gene Target Identification & Pathway Analysis, TruSeq Methyl Capture EPIC Library Prep Kit, Genetic Contributions of Cognitive Control, Challenges and Potential of NGS in Oncology Testing, Partnerships Catalyze Patient Access to Genomic Testing, Patients with Challenging Cancers to Benefit from Sequencing, NIPT vs Traditional Aneuploidy Screening Methods, SNP Array Identifies Inherited Genetic Disorder Contributing to IVF Failures, NIPT Delivers Sigh of Relief to Expectant Mother, Education is Key to Noninvasive Prenatal Testing, Study Takes a Look at Fetal Chromosomal Abnormalities, Rare Disease Variants in Infants with Undiagnosed Disease, A Genetic Data Matchmaking Service for Researchers, Using NGS to Study Rare Undiagnosed Genetic Disease, Progress for Patients with Rare and Undiagnosed Genetic Diseases. We developed a metagenomic next-generation sequencing (mNGS) test using cell-free DNA from body fluids to identify pathogens. 16s & ITS rRNA Sequencing; Metagenomic Sequencing; Microbial Whole-Genome Sequencing; Microbial Transcriptomics; Infectious Diseases. These sequencing reagent kits offer increased stability and robustness over the v2 kit version. Analysis, Biological Data In addition to producing twice the number of sequencing reads, this method enables more accurate read alignment and detection of structural rearrangements. There is also a 150-cycle format that enables counting applications. My experience as an intern at Illumina was simply fantastic. Nearly all infectious agents contain DNA or RNA genomes, making sequencing an attractive approach for pathogen detection. (For example, a 300-cycle kit can be used for a 1 300 bp single-read run or a 2 150 bp paired-end run.). It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. Collaborate with Illumina moderators, customers, and developers. We developed a metagenomic next-generation sequencing (mNGS) test using cell-free DNA from body fluids to identify pathogens. The MiSeq benchtop sequencer enables targeted and microbial genome applications, with high-quality sequencing, simple data analysis, and cloud storage. from 1 ng to 100 ng, depending on the application needs. Sequence Hub, BaseSpace Targeted custom research panels optimized for sequencing specific targets or genomic content of interest. Apps, DRAGEN limiting, such as blood, cell culture, or fresh-frozen tissues as well as challenging sample types, If you currently own a MiSeq, you may be eligible for a trade-in to a new NextSeq 1000 or NextSeq 2000. The iGenomes are a collection of reference sequences and annotation files for commonly analyzed organisms. Tax Reg: 105-87-87282 | As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. Bio-IT Platform, TruSight Variant Interpreter, MyIllumina rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics. This process translates into sequencing hundreds to thousands of genes at one time. | The human exome represents less than 2% of the genome, but contains ~85% of known disease-related variants, 1 making this method a cost-effective alternative to whole-genome sequencing. 16s & ITS rRNA Sequencing; Metagenomic Sequencing; Microbial Whole-Genome Sequencing; Microbial Transcriptomics; Infectious Diseases. AmpliSeq for Illumina is a suite of AmpliSeq chemistry products that are compatible with Illumina next-generation sequencing platforms. For more information, see the iGenomes Overview. The MiSeq v3 kits can achieve double the amount of output per flow cell. There are two sequencing read types: single-read and paired-end sequencing. Oligonucleotide (oligo) sequences of Illumina adapters used in AmpliSeq, Nextera, TruSeq, and 16s & ITS rRNA Sequencing; Metagenomic Sequencing; Microbial Whole-Genome Sequencing; we strive to meet this challenge. Ribosomal Database Project at Michigan State University. Reagents for 96 reactions, resulting in fast amplicon library preparation with AmpliSeq panels. All trademarks are the property of Illumina, Inc. or their respective owners. rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. The performance of mNGS testing of 182 body fluids from 160 patients with acute illness was evaluated using two sequencing platforms in comparison to microbiological testing using culture, 16S bacterial PCR and/or 28S-internal While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene The 16S gene copy number data is provided by rrnDB website.. We are pleased to release two new Fungal ITS training sets to classify fungal ITS Illumina sequencing and array technologies fuel advancements in life science research, translational and consumer genomics, and molecular diagnostics. The files have been downloaded from Ensembl, NCBI, or UCSC. For specific trademark information, see emea.illumina.com/company/legal.html. Each iGenome is available as a compressed file that contains sequences and annotation files for a single genomic build of an organism. The human exome represents less than 2% of the genome, but contains ~85% of known disease-related variants, 1 making this method a cost-effective alternative to whole-genome sequencing. Sequence Hub, BaseSpace Host: https://www.illumina.com | The Lander/Waterman equation1 is a method for calculating coverage (C) based on your read length (L), number of reads (N), and haploid genome length (G): C = LN / G. Different RNA-Seq experiment types have unique sequencing read length and depth requirements. For specific trademark information, see www.illumina.com/company/legal.html. Interpretation, Certificates (CofC, CofA) and Master Lot Sheets, AmpliSeq for Illumina Cancer Hotspot Panel v2, AmpliSeq for Illumina Comprehensive Cancer Panel, Breast Cancer Target Identification with High-Throughput NGS, The Complex World of Pan-Cancer Biomarkers, Microbiome Studies Help Refine Drug Discovery, Identifying Multidrug-Resistant Tuberculosis Strains, Investigating the Mysterious World of Microbes, IDbyDNA Partnership on NGS Infectious Disease Solutions, Infinium iSelect Custom Genotyping BeadChips, 2020 Agricultural Greater Good Grant Winner, 2019 Agricultural Greater Good Grant Winner, Gene Target Identification & Pathway Analysis, TruSeq Methyl Capture EPIC Library Prep Kit, Genetic Contributions of Cognitive Control, Challenges and Potential of NGS in Oncology Testing, Partnerships Catalyze Patient Access to Genomic Testing, Patients with Challenging Cancers to Benefit from Sequencing, NIPT vs Traditional Aneuploidy Screening Methods, SNP Array Identifies Inherited Genetic Disorder Contributing to IVF Failures, NIPT Delivers Sigh of Relief to Expectant Mother, Education is Key to Noninvasive Prenatal Testing, Study Takes a Look at Fetal Chromosomal Abnormalities, Rare Disease Variants in Infants with Undiagnosed Disease, A Genetic Data Matchmaking Service for Researchers, Using NGS to Study Rare Undiagnosed Genetic Disease, Progress for Patients with Rare and Undiagnosed Genetic Diseases. For other applications, such as expression profiling or counting studies, shorter reads are sufficient and little as 1 ng of input. The team at HudsonAlpha have been innovators in integrating genome sequencing into medicine. Read more about how to achieve consistent cluster density in order to streamline data outputs. This tool helps you estimate sequencing coverage and contains read length recommendations for your experiment. Read the latest bulletin to learn more about your maximum read length along with other resources to optimize data quality. rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. 16S and Internal Transcribed Spacer (ITS) ribosomal RNA (rRNA) sequencing are common amplicon sequencing methods used to identify and compare bacteria or fungi present within a given sample. Made-to-order panels containing content selected and designed with input from leading disease researchers. User-friendly Illumina tools smooth the process of analyzing sequencing data, so you can spend more time doing research and less time configuring workflows. The fast and simple protocol allows for treatment of one or a pool of libraries just prior to sequencing on any Illumina platform. * In addition to our industry-leading data quality, Illumina offers integrated mRNA-Seq workflows that simplify the entire process, from library preparation to data analysis and biological interpretation. Tax Reg: 105-87-87282 | Targeted DNA and RNA research panel investigating 52 genes with known relevance to solid tumors. 16S and Internal Transcribed Spacer (ITS) ribosomal RNA (rRNA) sequencing are common amplicon sequencing methods used to identify and compare bacteria or fungi present within a given sample. ChIP-Seq may require only a few reads (~5-15 million) for a highly targeted transcription factor, and many more reads (~50 million) for a ubiquitous Join Now 16s & ITS rRNA Sequencing; Metagenomic Sequencing; Microbial Whole-Genome Sequencing; Microbial Transcriptomics; Infectious Diseases. 2022 Illumina, Inc. All rights reserved. Available in convenient, small pack sizes of 24 or 96 reactions. Illumina Korea 14F KTB Building 66 Yeoidaero Yeoungdeungpo-gu Seoul Korea 07325 MiSeq Regent Kits v2 are available in a 500-cycle format for long read lengths, as well as in the popular 50-cycle and 300-cycle formats. Keep samples and data in house, for more control and ability to build a database; A future-proof assay with emerging biomarkers already included; Learn more about the TruSight Oncology Product Family For specific trademark information, see www.illumina.com/company/legal.html. Learn how AmpliSeq for Illumina panels provide a simple, fast, and robust sequencing option to Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million nonredundant microbial genes, derived from 576.7 Gb sequence, from faecal samples of 124 European individuals. polymerase chain reaction (PCR)-based workflow for use with targets ranging from a few to hundreds Retailer Reg: 2019--2018 |
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